Analysis Note

ControlHepG2 cell lysate

Application

Detect Dlx 1 using this mouse monoclonal antibody, Anti-DLX-1 Antibody, clone L43/40 validated for use in western blotting & IHC.

Research CategoryNeuroscience

Immunohistochemistry Analysis: A representative lot detected DLX-1 in rat hippocampus tissue (Prof. J. Trimmer, University of California, Davis.).

Western Blotting Analysis: A representative lot detected DLX-1 in whole cell extracts of wild type embryonic day 15 telencephalons and demonstrated a loss of signal in whole cell extracts of embryonic day 15 telencephalons from DLX1/2 knock out mice (Prof. J. Trimmer, University of California, Davis.).

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Homeobox protein DLX-1 (DLX-1) is a developmental potein that is localized within the cell nucleus. DLX-1 likely has regulatory roles in the development of the ventral forebrain, craniofacial patterning and morphogenesis, and may be involved in the early development of diencephalic subdivisions.

Immunogen

Linear peptide corresponding to human DLX-1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HepG2 cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected DLX-1 in 10 µg of HepG2 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~27 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.